Disc 1

1. Mississippi Sheiks - Sittin' On Top of the World 3.02
2. Mississippi John Hurt - Staggolee 2.55
3. Big Maceo - Worried Life Blues 2.54
4. Bessie Smith - Nobody Knows You When You're Down and Out 2.56
5. Art Tatum - St. Louis Blues 2.35
6. Charlie Patton - High Sheriff Blues 3.07
7. Leroy Carr & Scrapper Blackwell - How Long, How Long Blues 3.03
8. Lonnie Johnson - Got the Blues for Murder Only 3.22
9. Louis Armstrong - St. James Infirmary 3.12
10. Tampa Red & Georgia Tom - You Can't Get That Stuff No More 2.56
11. Big Bill Broonzy - Long Tall Mama 2.46
12. Blind Boy Fuller - Rag Mama Rag 3.03
13. Curtis Jones - Moonlight Lover Blues 2.32
14. Jazz Gillum - Early in the Morning 2.51
15. Lucille Bogan - Shave 'Em Dry 2.49
16. Blind Willie McTell - Georgia Rag 3.02
17. Meade Lux Lewis & Pete Johnson - Boogie Woogie Prayer (Part 1) 2.20

Disc 2

1. Robert Johnson - 32-20 Blues 2.48
2. Son House - Death Letter Blues 4.19
3. Memphis Minnie - Me & My Chauffeur Blues 2.45
4. Billie Holiday - Fine and Mellow 6.21
5. Duke Ellington - Mississippi Moan 3.24
6. Memphis Slim - Whiskey & Gin Blues 2.41
7. Champion Jack Dupree - Dupree Shake Dance 2.41
8. Robert Lee McCoy - Take It Easy Baby 2.30
9. Joe Williams - Baby, Please Don't Go 2.47
10. Bukka White - Fixin' to Die 2.46
11. Leadbelly - Easy Rider 3.11
12. Sonny Boy Williamson - Sloppy Drunk Blues 3.13
13. Taj Mahal - Texas Woman Blues 2.55
14. Willie Dixon - You Shook Me 4.15
15. Larry Williams & Johnny Watson - Summertime (Instrumental) 3.10
16. Roy Milton - R.M. Blues 3.02
17. Muddy Waters - I Can't Be Satisfied 3.29

Disc 3

1. Muddy Waters - Mannish Boy 5.24
2. Otis Spann - Ain't Nobody's Business 5.12
3. Buddy Guy - Lucy Mae Blues 3.33
4. Fleetwood Mac - Black Magic Woman 2.46
5. Johnny 'Guitar' Watson & Larry Williams - Unchain My Heart 3.14
6. Michael Bloomfield - Mary Ann 5.28
7. The Fabulous Thunderbirds - Down at Antone's 3.03
8. Johnny Winter - Mean Town Blues 8.25
9. Chris Whitley - Poison Girl 3.25
10. Stevie Ray Vaughan - Cold Shot 4.01
11. Jimmie Vaughan - Motor Head Baby 4.00
12. Electric Flag - Texas (Feat. Buddy Miles) 4.46
13. Taj Mahal - Chevrolet 2.37
14. Taj Mahal - Chevrolet 2.37
15. Bob Dylan - Dirt Road Blues 3.35
16. Bob Dylan - Dirt Road Blues 3.35
17. Keb' Mo' - Am I Wrong 2.18
18. Keb' Mo' - Am I Wrong 2.18
19. Alvin Youngblood Hart - Gallows Pole 4.45
20. Alvin Youngblood Hart - Gallows Pole 4.45